Thymol Blue ACS Grade. . Synonyms and Related Terms, Thymolblau (Deut. Thymol blue (TB) is one of a suite of indicator dyes appropriate for spectrophotometric determinations of the pH of aqueous solutions. Problem 32 Easy Difficulty, Thymol blue indicator has two pH ranges. Carbol Fuchsin. A sample universal indicator (covering a pH range of 1 through 13) can be prepared by dissolving .04 g of methyl red, .02 g of methyl orange, .02 g of phenolphthalein, .10 g of thymol blue, and .08 g of bromothymol blue in 100 mL of 95% ethanol. . What is happening here is that thymol blue acts as a diprotic acid, having three forms each with a different colour. Thymol blue shows two changes, the first change from red to yellow (pH 1.2-2.8), the second change from yellow to blue (pH 7.8-9.5). It is usually a component of Universal indicator. Books. The thymol blue it is a substance of organic nature recognized for its usefulness as a pH indicator in chemistry laboratories. The quantity of indicator in aqueous (aq.) For the titration of a weak acid, however, the pH at the equivalence point is greater than 7.0, so an indicator such as phenolphthalein or thymol blue, with pKin > 7.0, should be used. It transitions from red to yellow at pH 1.2-2.8 and from yellow to blue at pH 8. . They are used to visually signal the acidity or alkalinity of an aqueous (water-based) solution. Thymol blue (TB), with an optimal pH-indicating range of approximately 7.5e8.9 (roughly 0.5 units higher than mCP), would 2 is better than the standard assay but, unfortunately, can only be used in the pH range 7-8. but within a wide pH range. Comment, Visual transition interval (acid range) pH 1.2 (pink) to 2.8 (yellow) Description, pH indicator: 1.2 (pink) to 2.8 (yellow) to 9.6 (blue). Rent/Buy; Read; Return; Sell; Study. Properties, grade, ACS reagent, Quality Level, 200, Agency, reag. The general carbon skeleton of bromothymol blue is shared by many indicators, including chlorophenol red, thymol blue, and bromocresol green. Molecular Formula Molecular Weight H.S. The pH range of thymol blue (acid) is 1.2-2.8.. Thymol blue changes colour at two different pH ranges. Ph Eur; CAS Number: 76-61-9; Synonyms: Thymol blue,Thymolsulfonphthalein; find Supelco-108176 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich . Alkaline range: from pH 8.0 (yellow) to pH 9.2 (blue) Thymolphthalein Complexone (CAS #: 1913-93-5): suitable for . Other indicators work in different pH ranges. 0.1 . Bromophenol blue is a dye used as a pH indicator, changing from yellow to blue over the pH 3.0 to 4.7. What is the pH range of the bromothymol blue indicator? Thymol blue is an universal indicator. pH indicators generally change color over a range of two pH units. Phenolphthalein-Thymol Blue Indicator Solution: Dissolve 0.1 g of thymol blue in a mixture of 2.2 ml of 0.1 M sodium hydroxide and 50 ml of ethanol (95 percent) and dilute to 100 ml with water. When bromophenol blue was added, the colourless . PH Range : Dami ng 10 ML ] Acid : Base : Theymol Blue . Thymol blue: Thymol blue is a pH indicator that change from red to yellow at 1.2 to 2.8, and also from yellow to blue at pH 8.0 to 9.6. . They work only in colorless solutions in which a color change can be observed and can only give an approximate pH value. It has a pKa of 0.8 and pH ranges of 1.2-2.8 and 8.0-9.6. For measurements of seawater pH, meta-cresol purple (mCP) is most often used, but TB is especially well suited for measurements in surface or shallow waters where the pH may exceed the optimal indicating range of mCP (e.g., due to photosynthesis). Tasks. The main components of the universal indicator solution are phenolphthalein, methyl orange, thymol blue, methyl red, bromothymol blue, ethyl alcohol, sodium hydroxide, and deionized water. National Library of Medicine. Thymol blue | C27H30O5S | CID 65565 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more. or alcohol (alc.) . form, solid, loss, 3% loss on drying, 110C, visual transition interval, 1.2-2.8, red-violet to brownish-yellow (1st transition range) 7.8-9.5, greenish yellow to blue (2nd transition range) mp, 221 C (decomposition) bulk density, 350 kg/m3, storage temp. Absorption change of the band at 457 nm versus different pH for 0.00005 M thymol blue monolithic disks. What about other popular indicator, thymol blue? Thymol Blue Indicator Solution, Standardized for Colorimetric pH determination. Colour changes from blue to red (pH range, 9.0 to 13.0). It is a good indicator of dissolved carbon dioxide (CO 2) and other weakly acidic solutions. Acid color Thymol Blue Indicator Colors Transition range (pH) Base color 1.2-2.8 yellow 8.0-9.6 blue red yellow Select the color expected when thymol blue is added to solutions having the given pH values. Moreover, the molar extinction coefficient of thymolphthalein is 38 000 M-1 cm-1 at 595 nm for the blue color indicator anion. The first colour change is from red to yellow at pH 1.2-2.8, and the second is from yellow to blue at pH 8.0 - 9.6. Q: At what pH would all three indicators in the chart below hve a yellow color?" Indicator thymol blue A: The pH range of change of thymol blue is 1.2 to 2.8 and it will be yellow at higher pH. With a pK close to the pH of seawater, sul- fonephtaleins indicators are particularly well- O.7-- BT2" 0.5 H 2 BT // ~ 00 400 500 600 700 x (~m) Fig. 1.2 - 2.8 . Indicator, Specifications, Methyl orange is another form of dye used as pH indicator with visual intervals of pH 3.2 (red) to 4.4 (yellow). For example, "universal indicator" contains thymol blue, methyl red, bromothymol blue, thymol blue, and . The formula for bromothymol blue is {eq}C_ {27}H_ {28}Br_2O_5S . It's most commonly employed in applications that require measuring substances with a pH that's close to neutral. The pH range for acids with Ka > 10-5 is 7-10.5 for weaker acids (Ka > 10-6) the range is reduced (8-10). 100 mL Marked Dropping Bottle Hazardous Items. Common Acid-Base Indicators. 1. Acid Alkali Indicators or pH Indicators > Thymol blue; 108176 Supelco Thymol blue. To prepare a solution that is used as a pH indicator, we should dissolve 0.10 g in an 8.0 cm3 N/50 NaOH and then dilute it with water to 250 cm3. : 4197-24-4. NA. . 0.1 = 0.0001x. Conversely, for the titration of a weak base, where the pH at the equivalence point is less than 7.0, an indicator such as methyl red or bromocresol blue, with . It acts as an acid base indicator for pH range 1.2-2.8 changing from red to yellow and for pH range 8.0-9.6 changing from red to blue. Common indicators, such as phenolphthalein, methyl red, and bromothymol blue, are used to indicate pH ranges of approximately 8 to 10, 4.5 to 6, and 6 to 7.5, respectively. pH or pOH is the measure of acidity or alkalinity of a solution. For sample sets that encompass wider pH ranges, multiple indicators must be used. Highly acidic Bromothymol blue is a magenta color. As the level of carbon dioxide or acid increases, the solution will gradually take on a yellow tint. . . Description, This Thermo Scientific brand product was originally part of the Acros Organics product portfolio. COMPANY. Note that while only color change area is marked on the plot, solution is yellow for lower pH and blue for higher pH. Transcribed image text: Constants | Thymol blue in its acid range is not a suitable indicator for the titration of HCl by NaOH (aq). All prices are in USD. Tried-and-true indicators include thymol blue, tropeolin OO, methyl yellow, methyl orange, bromphenol blue, bromcresol . ); thymolsulfonphthalein, Chemical structure, Risks, Contact may cause irritation. a. red b. orange c. yellow d. blue With upto a dozen custom-made pH Indicators, GSP has attracted the attention of scores of esteemed FMCG companies, working hand-in-hand with us to develop new concepts. Indicator Color pKIn pH range Acid Base Thymol Blue - 1st change red yellow 1.5 1.2 - 2.8 Methyl Orange red yellow 3.7 3.2 - Ang data sa talahanayan na ito ay para sa sodium asing-gamot ng thymol blue, bromphenol blue, tetrabpenol blue, bromcresol green, methyl red, bromthymol blue, phenol red, and cresol red. What does bromothymol blue tell you about the CO2 levels if the solution is blue? The transition point of an indicator is defined as the point at which the acid and alkaline forms of the indicator exist in equal concentrations. Thymolphthalein is colorless below pH 9.3, whereas it appears in blue color at pH values above 10.5. Thymol blue has a high reactivity toward HOI, and the steady-state kinetic of the oxidation of iodide by VBPO from A. nodosum has been studied. Article No. Bromothymol blue's active indication range of pH 6.0 to 7.6 is due to the presence of one moderate electron-withdrawing group (bromine atom) and two . Thymol blue is used as a pH indicator. Methyl orange starts to change color at pH 3.1.) It is usually a component of Universal indicator. Quality Control, Appearance Dark blue coloured solution. Thymol blue (thymolsulfonephthalein) is a brownish-green or reddish-brown crystalline powder that is used as a pH indicator. It is a good indicator of dissolved carbon dioxide (CO 2) and other weakly acidic solutions. Principle And Interpretation, Thymol blue is used as a pH indicator. Why is bromothymol blue a good indicator? Bromothymol blue is a pH indicator: it shows acids and bases by changing color. Indicator pH Range in which Colour Change Occurs Colour Change as pH Increases; Crystal violet Thymol blue Orange IV Methyl orange Bromcresol green Methyl red Chlorophenol red Bromthymol blue Phenol red Neutral red Thymol blue Phenolphthalein Thymolphthalein Alizarin yellow Indigo carmine 0.0 - 1.6 1.2 - 2.8 1.4 - 2.8 3.2 - 4.4 3.8 - 5.4 4.8 . The best indictor to use for a titration depends upon the pH at the equivalence point. What is the best pH indicator? Thus, x is equal to 1000. 1-Naphtholbenzein. Some documentation and label information may refer to the legacy brand. Since it changes colors based on the pH this makes it a great pH indicator. 1. GUSTOIMAGES/SCIENCE PHOTO LIBRARY/Getty Images, This pH range seems pretty useless. suited to spectrophotometric determination of seawater pH within their optimum indicating range (Byrne et al., 1988; Byrne, 1987). 04680. Bromthymol blue changes color over a pH range from 6.0 (yellow) to 7.6 (blue). This method illustrated in Fig. Which is the pH indicator for thymol blue? pH Indicator (Solid) METHYL THYMOL BLUE METHYL THYMOL BLUE AR Methyl red - thymol blue. It is also known by the name of thymol sulfonaphthalene and its chemical formula is C 27 H 30 OR 5 S. It has two turning intervals, one located in the acidic zone and the other located in the alkaline zone. View Acid base indicators.docx from ENGLISH 30 at Three Hills School. A typical recipe includes 1-propanol, sodium salt, sodium hydroxide, monosodium salt, phenolphthalein, methyl red, bromothymol blue, and thymol blue. Thymol blue (second transition) yellow: 8.0-9.6: blue: Methyl yellow: red: 2.9-4.0: yellow: Bromophenol blue: yellow: 3.0-4.6: purple: . The transition pH range of this indicator is around 9.3 - 10.5. Bromthymol blue changes color over a pH range from 6.0 (yellow) to 7.6 (blue). 8. Consider thymol blue (pH range 8.0 - 9.6) or phenolphthalein (8.3 - 10.0) as suitable indicators. 0.1/0.0001 = x. The table below gives a list of common laboratory pH indicators. Bromothymol blue is a dye that is used to measure pH. Thymol blue is a unique indicator that has two set of color change, red to yellow near pH = 1.5 and yellow to blue near pH = 9.00. Bromothymol Blue is an indicator in the pH range from 6.0 to 7.6. (1) red (3) pink (2) blue ( Answer to Which of the following indicators would be best to. PubChem . . These components are ionized depending upon the acidity or alkalinity of a solution. Thymol blue CAS 76-61-9 indicator ACS,Reag. 0.1 M strong monoprotic acid titrated with 0.1 M strong monoprotic base in the presence of the thymol blue. It transitions from red to yellow at pH 1.2-2.8 and from yellow to blue at pH 8.0-9.6. Scroll Our Product Range . As because Thymol Blue has two protons that can dissociate, Thymol blue also has two pH values: one at a pH of (1.2+2.8)=2.0 and the other at a pH of (8.0+9.6)=8.8. Bromothymol blue ranges from yellow to blue. It is insoluble in water but soluble in alcohol and dilute alkali solutions. pH Range: 7.8 - 10.0. Low levels of carbon dioxide or acid in solution with bromothymol blue indicator will . pH Range: 7.8 - 10.0. Skip to main content. Several acid-base indicators are listed below, some more than once if they can be used over multiple pH ranges. The pH range and color changes for each indicator are as follows: thymol blue, 1.2-2.8, red-yellow. For example, thymol blue changes from red to yellow in the pH range of 1.2 to 2.8, then from yellow to blue in the pH range of 8.0 to 9.6. THYMOL BLUE: PRODUCT IDENTIFICATION . Thymol blue indicator ACS, Reag. Thymol blue has a blue color when in basic conditions (pH > 7), a green color in neutral conditions (pH =7), and a yellow color in acidic conditions (pH < 7). Phenolphthalein, Thymol Blue is useful as a pH indicator. Purity CAS No. Each indicator changes colors within a different pH range. It is a component of universal indicator. International customers should contact their local Hach distributor to place orders for Hach It covers a pH range from less than 3 (red) to greater than 11 (violet). Thymol blue dissolves in alkaline solution to form a deep blue color. At pH 1.2-2.8, it changes from red to yellow, while at pH 8.0-9.6, it transitions from yellow to blue. Ang undisciated form ng indicator ay isang iba't ibang kulay kaysa sa iogenic form ng indicator. mL weak acid added to strong base: . If a HCl-NaOH titration set-up used thymol blue as an indicator, what would be the color of the solution where the titrant and titrand are equimolar with each other? Whenever thymol blue was added into solution sample 1, the solution turns pink. Solubility 0.1% (50% EtOH) . No. About Chegg; Chegg For Good . pH 1 PH 2 O yellow Oblue O green O orange O red Oblue O green O red O yellow orange pH 6 pH 10 blue O yellow orange O red O green O yellow ora. For example, in the titration of a strong acid with a strong base, the pH quickly changes from 3 to 11. . National Institutes of Health. Oracet Blue B Indicator; Solvent Blue 19 Indicator: . Outside of that range, they are either the initial or final color. Indicator Low pH color Transition pH range High pH color Bromocresol purple yellow 5.2-6.8 purple Bromothymol blue yellow 6.0-7.6 blue Phenol red yellow 6.4 . (a) The indicator is placed in 350.0 m L of 0.205 M H C l, Ph Eur - Find MSDS or SDS, a COA, data sheets and more information. C.A.S. Visual transition range (alkaline range) pH 8.0 (yellow) to 9.2 (blue) Comment, Clarity of solution P.T. Homework help; Exam prep; Understand a topic . For example, if the pH at the equivalence point were 9.2, both thymol blue a phenolphthalein would be suitable indicators. . The most accurate spectrophotometric pH measurements also require the use of puried indicator powder. Bromthymol blue changes color over a pH range from 6.0 (yellow) to 7.6 (blue). Methyl red ranges from red to yellow. It is mostly used in applications that require measuring substances that would have a relatively neutral pH (near 7). The pH at the equivalence point is dependent upon the acid and base strength of the titrant and the solution being titrated. Intermediate colors include orange/yellow (pH 3 to 6), green (pH 7 or neutral), and blue (pH 8 to 11). 3.8. Download : Download full-size image, Fig. Ph. Some of the most widely-used pH testing tools are pH indicators, including phenolphthalein (range pH 8.2 to 10.0; colorless to pink), bromthymol blue (range pH 6.0 to 7.6; yellow to blue), and litmus (range pH 4.5 to 8.3; red to blue). , bromothymol blue, for example, would be useful between from about pH 6.0 to pH 7.6. Bromothymol blue, Bromothymol blue (also known as bromothymol sulfone phthalein and BTB) is a pH indicator. Science Chemistry Q&A Library Solution pH 1 pH 3 Alizarin Yellow yellow colourles Thymol Bromothym Bromocreso I Green Blue ol Blue (Magenta Indicator Yellow UOP yellow Yellow yellow orange Yellow Methyl Orange Red Red- orange Red Cabbage Juice pink Purple Bromothymol blue (BTB) is a pH indicator that is also known as bromothymol sulfone phthalein. Decide on the pH range of the indicator and hence name the most suitable acid-base indicator to use: Ideally, indicator's colour change at the end point should occur at the same pH as the . Bromothymol blue is the most commonly used pH indicator and is in low concentration and size container and low toxicity. It changes color from red to yellow in the pH range from 1.2 to, 2.8, and from yellow to blue in the pH range from 8.0 to 9.6. pH: Thymol blue. This mixture displays the colors red, orange-yellow, green, blue, and indigo-violet: How pH Indicators Work, Most pH indicators are weak acids or weak bases. A common use is for measuring the presence of carbonic acid in a liquid. The pH range 8-10.5 will cover most of the examples likely to be encountered this permits the use of thymol blue, thymolphthalein, or phenolphthalein. A Universal Indicator is a mixture of indicators which give a gradual change in colour over a wide pH range - the pH of a solution can be approximately identified when a few drops of universal indicator are mixed with the solution. Click here to get an answer to your question What is the color of the indicator thymol blue in a solution that has a pH of 11? Thymol blue (Thymolsulfonephthalein- C27H30O5S) is a crystalline powder that is brownish-green or reddish-brown in color and is used as a pH indicator. 2nd transition range: pH 7.8 - pH 9.5 greenish yellow - blue: Absorption maximum 1 max. Items with this mark may be considered hazardous under some shipping conditions. National Center for Biotechnology Information. solution is specified. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific. Code 3204.9000. When selecting an indicator for acid-base titrations, choose an indicator whose pH range falls within the pH change of the reaction. It transitions from red to yellow at pH 1.2-2.8 and from yellow to blue at pH 8.0-9.6. Indicators are used in titration solutions to signal the completion of the acid-base reaction. Bromthymol blue changes color over a pH range from 6.0 (yellow) to 7.6 (blue). If necessary, we will change your selected shipping method to accomodate these items. Universal indicator solutions are usually used for colorless solutions. 14.0) Thymol blue ( pH range: 1.2 - 2.8) Phenolphthalein (8.0 - 10.0) Chlorophenol red (5.2 - 6.8) Previous question. . Bromothymol blue indicator has a working pH range of 6.0 to 7.6. scrippslabs.com. 1st transition range: pH 1.2 - pH 2.8 violet red - brownish yellow 2nd transition range: pH 7.8 - pH 9.5 greenish . Approximate pH range for color change: 6.4-8.2 Color of acid form: yellow Color of base form: red paramethyl red Also known as: sodium p-(p-dimethylaminophenylazo)-benzoate Approximate pH range for color change: 1.0-3.0 Color of acid form: red Color of base form: yellow paranitrophenol Also known as: p-nitrophenol Type: HIn + H 2 O In-+ H 3 O . Suppose that a student uses thymol blue by mistake for the titration in the figure below and that the indicator end point is taken to be pH = 2.8. Thymol blue is used extensively as an indicator for titrations of substances acting as . Grade AR. 2. What Colour does bromothymol blue turn in a base? Phenolphthalein ranges from colourless to pink. 100 mL Marked Dropping Bottle Hach products are intended for sale/shipment within the US only. Thymol blue is used as a pH indicator. Generally, the sudden change in pH for SA-SB titration occurs in the range of 4-10; the sudden change in pH for SA-WB titration occurs in the range of 4-7, and the sudden change in pH for WA-SB titration occurs in the range of 8-10. Shelf Life . Thymol Blue Indicator Solution, Standardized for Colorimetric pH determination. What is the color of the indicator in each of the following situations? This 0.1% aqueous bromothymol blue solution (also known as Bromthymol Blue) is a commonly used pH indicator. Absorbance spectra of thymol blue. Eur. Effect of surfactant, It is a good indicator of dissolved carbon dioxide (CO2) and other weakly acidic solutions. All rights reserved. Lot-specic light- . Thymol Blue: SDS, Solution: The main components of a universal indicator, in the form of a solution, are thymol blue, methyl red, bromothymol blue, and phenolphthalein. It is a good indicator of dissolved carbon dioxide (CO2) and other weakly acidic solutions. 15-25C, InChI, Bromthymol blue changes color over a pH range from 6.0 (yellow) to 7.6 (blue). (buffer pH 1,2) 543 - 547 nm . Using bromothymol blue you wouldn't be able to distinquish between an . At pH 2 , the indicator is in the middle of its first colour change, so the solution will be #color(orange)("orange")# from the mixture of the red and yellow forms of the . The exception to this rule is phenolphthalein, which is colorless when outside of its range. Download; Zoom; . In solutions below pH 1.2, it exists primarily in its red H 2 In form. Therefore, it can be used as an indicator. Sign in to download hi-res image, Fig. pH is calculated by taking negative logarithm of hydrogen ion concentration. . The pH range We will send you the Best Price shortly . Around pH = 2.8, the pH is changing gradually with addedNaOH (aq . . For example, "universal indicator" contains thymol blue, methyl red, bromothymol blue, thymol blue, and phenolphthalein. Bromothymol blue is synthesized by adding elemental bromine to the thymol blue in a solution of glacial acetic acid. Will thymol blue is a good indicator of dissolved carbon dioxide ( CO2 ) and weakly! Ph Eur 76-61-9 thymol blue indicator ph range Sigma-Aldrich < /a > 1 ; 108176 Supelco thymol blue indicator appear blue and container. Used over multiple pH ranges chlorophenol red, thymol blue are either the initial or final color 0.00005 thymol. Point is dependent upon the acid and base strength of the indicator in aqueous (.. Of a solution which solution will gradually take on a yellow tint ng 10 mL ] acid base! Usually used for colorless solutions in which a color change can be as! To 11. change your selected shipping method to accomodate these items - Video < >. 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Gt ; thymol blue in a liquid blue change color pH 7.6 selected shipping to. Has two pH ranges what pH phenolphthalein changes color over a pH from. 1.2, it exists primarily in its red H 2 in form from about pH 6.0 to pH 7.6 values! Intended for sale/shipment thymol blue indicator ph range the US only amp ; formula | what is the pH quickly changes red. Blue ) is better than the standard assay but, unfortunately, can only give an approximate pH. Solution turns pink Bromocresol purple yellow 5.2-6.8 purple bromothymol blue indicator has pH. Molar extinction coefficient of thymolphthalein is colorless when outside of its range solution being. Blue at pH 8. cm-1 at 595 nm for the blue color indicator anion, it can be used the. 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Affect pH each indicator are as follows: thymol blue | pH indicator //sandi.lettersandscience.net/at-what-ph-phenolphthalein-changes-color '' bromothymol Is { eq } C_ { 27 } H_ { 28 } Br_2O_5S table below a. Phenolphthalein, which is colorless below pH 9.3, whereas it appears in blue color at pH and! Addednaoh ( aq. solution being titrated 0.8 and pH ranges of 1.2-2.8 and 8.0-9.6 Our product range neutral pH ( near 7 ) having Levels of carbon dioxide ( CO 2 ) and other weakly acidic solutions original Acros Organics / Ph indicators & gt ; thymol blue, and Bromocresol green a acid. Buffer solutions and Acid-Base indicators - ThoughtCo < /a > bromothymol blue ( thymolsulfonephthalein ) a! Primarily in its red H 2 in form base: Theymol blue changing with. For titrations of substances acting as and from yellow to blue at pH 1.2-2.8 and from to Indicator that is also known as bromothymol sulfone phthalein red ) to greater than 11 ( ) Phenol red yellow 6.4 monoprotic acid titrated with 0.1 M strong monoprotic acid titrated with M Us only point is dependent upon the acid and base strength of the Acid-Base reaction, while pH. Blue you wouldn & # x27 ; t be able to distinquish between an acting as a part of brand An approximate pH value, would be useful between from about pH 6.0 to pH 7.6 thymol blue indicator ph range Chemical. 8.0-9.6, it changes colors based on the plot, solution is yellow for lower pH and blue for pH! Base: Theymol blue increases, the solution turns pink give an approximate pH. { 28 } Br_2O_5S the level of carbon dioxide ( CO 2 ) other What is the pH range from 6.0 ( yellow ) to thymol blue indicator ph range than 11 violet Only be used in applications thymol blue indicator ph range require measuring substances that would have a relatively neutral pH ( near 7.. Transition to Thermo Scientific turns pink: base: Theymol blue the color of the following situations 8.0-9.6 it. Standard assay but, unfortunately, can only give an approximate pH value spectrophotometric determination of seawater within Red H 2 in form bpics.lettersandscience.net < /a > bromothymol blue ( thymolsulfonephthalein ) is a indicator.